Figures & data
Figure 1. Growth of the wild type (Wt), Cu chaperone mutants (cch, atx1 and cchatx1) and transgenic plants with overexpression of Arabidopsis Antioxidant Protein1 (ATX1) (Wt-ATX1–1, Wt-ATX1–2, atx1-ATX1–1 and atx1-ATX1–2) or Copper Chaperone (CCH) (Wt-CCH-1, Wt-CCH-2, cch-CCH-1 and cch-CCH-2) under severe Cu deficiency. A, Seeds of plants were grown vertically on half-strength MS agar plates with 50 μM Cu chelator bathocuproine disulfonate (BCS) for 17 d (A). Bar = 1 cm. Plants were grown in half-strength MS medium and treated with BCS 50 μM for 17 d, and fresh weight (B) and root length (C) were measured. Data are mean ± SD of 4 replicates with 40 seedlings each. Student’s t-test was used for statistical analysis. * p < 0.01 compared with the wild type under the same condition. The plant materials and growth conditions were described previously.Citation6
