A new DEAD-box helicase ATP-binding protein (OsABP) from rice is responsive to abiotic stress

Figures & data

Figure 1. Schematic representation of the OsABP (LOC_Os06 g33520) domain organization (A) and the presence of the nine conserved motifs in the DEAD-box helicase family (B). The numbers within brackets represent the amino acids present between the motifs.

Table 1. Percentage of similarity between OsABP and related RNA helicases form Arabidopsis thaliana and Oryza sativa.

UniProt Acc. No.	Protein name	Organism	Length (aa)	Identity %
Q0DBU5	DEAD-box ATP-dependent RNA helicase 31	Oryza sativa subsp japonica	574	93
Q9FNM7	DEAD-box ATP-dependent RNA helicase 26	Arabidopsis thaliana	850	51
Q0JL73	DEAD-box ATP-dependent RNA helicase 26	Oryza sativa subsp japonica	536	63
B9SBJ5	Dead box ATP-dependent RNA helicase	Ricinus communis	751	59
Q9FFQ1	DEAD-box ATP-dependent RNA helicase 31	Arabidopsis thaliana	761	58
Q94C75	DEAD-box ATP-dependent RNA helicase 25	Arabidopsis thaliana	563	62
Q5ZBH5	DEAD-box ATP-dependent RNA helicase 25	Oryza sativa subsp japonica	594	64

Figure 2. Schematic representation of the putative interaction of OsABP with other proteins. STRING program (http://string-db.org/) was used for the bioinformatic prediction of protein-protein interaction.

Figure 3. qRT-PCR analysis showing the expression levels of OsABP in two weeks-old rice plants grown in greenhouse and challenged with different abiotic stresses; (A) 200 mM NaCl, dehydration, 100 µM ABA, blue and red light, pH 3.5 and 10.5; (B) cold (4°C), heat (42°C) and while light; NT, non-treated. The relative expression is presented as fold change to control. Values are expressed as means ± SD of three independent replicated plants.