Figures & data
Figure 1. Expression of an integrated auxin-responsive reporter gene in Arabidopsis nnph4-1/arf7 protoplasts transfected with full-length or CTD truncated ARF5 and ARF7 proteins. Truncated constructs are denoted as 5D5M and 7D7M. Arabidopsis mesophyll protoplasts were isolated from nph4-1/arf7 leaves containing a stably integrated 2XD0:GUS (A) or DR5:GUS (B) reporter gene, full-length or CTD truncated ARF5 and ARF7 effector genes were transfected into protoplasts and incubated in darkness in the presence (black columns) and absence (white columns) of 1 μM 1-NAA (1-naphthalene acetic acid) for 20–22 h and then GUS activities were measured.Citation11 A 35S:CAT (chloramphenicol acetyltransferase) plasmid was used as a control for the amount of effector plasmid DNA (10 μg) introduced into protoplasts. Data represent mean ± SD of three replicates. In some case, error bars may not visible because of their small size.
Figure 2. Expression of an integrated 2XD0:GUS auxin-responsive reporter gene in Arabidopsis nph4-1/arf7 protoplasts transfected with CTD truncated ARF5 (5D5M) or the ARF5 DBD fused to ARF7 (5D7M) or VP16 AD (5DVP) or the ARF1 RD (5D1M). Assays were conducted as described in .
Figure 3. Expression of an integrated 2XD0:GUS auxin-responsive reporter gene in Arabidopsisnph4-1/arf7 protoplasts transfected with CTD truncated ARF5 (5D5M) or the ARF1 (1D5M), ARF6 (6D5M) or ARF7 DBD (7D5M) fused to ARF5 AD. Assays were conducted as described in .
Figure 4. Dose response for 1-NAA on the expression of an integrated 2XD0:GUS auxin-responsive reporter gene in Arabidopsisnph4-1/arf7 protoplasts transfected with full-length or CTD truncated ARF5 or the ARF5 DBD fused to the VP16 AD. Assays were conducted as described in using the concentration of 1-NAA indicated.
