Distinct salt-dependent effects impair Fremyella diplosiphon pigmentation and cellular shape

Figures & data

Figure 1. Accumulation of phycobiliprotein gene transcripts in cells grown with or without sodium chloride (NaCl) salt under green light (GL) or red light (RL). RT-PCR analyses of the expression of cpeA, cpeB, cpcA2 and cpcB2 in Fremyella diplosiphon grown with (200 mM) or without (0 mM) NaCl under GL or RL. The transcript level of the orf10B gene was used as an internal control for each sample. (A) Representative agarose gel images and (B) average transcript levels relative to orf10B (± SD) calculated using densitometry measurements of three independent biological replicates. Identical letters over bars represent a homogeneous mean group (p > 0.05) within the bars for a single gene.

Table 1. Sequence of primers used in this study

Primer	Sequence (5′-3′)	Annealing temperature (°C)
cpeA for cpeA rev	GCAACCGAAGCTTACAACG GCAGTAGGCAAGCCTAAAGC	51
cpcA2 for cpcA2 rev	GAGGGCGTAGCTACCGC CGTACCAGCTGGGAGACAG	53
cpeB for cpeB rev	ATGCTGTAGCTGGAATGATCTG CTGGGAGTGTCTTGAATGTGA	56
cpcB2 for cpcB2 rev	TAGAGGCGCATACATTAGCG GAACGCCTAATGCTTGATATGTTT	55
orf10B for orf10B rev	ATGGACACTACAGATAATTCCC TCATACATCAGAACGAAGGG	54