Figures & data
Figure 1. Characterization of rapamycin and FK506 sensitivity in WT, BP12–2, and BP12–5 plants. (A) Growth performance of WT, BP12–2, and BP12–5 plants grown on 0.5MS+DMSO, 100nM, 10nM, and 1nM rapamycin media for 25 d. WT plants showed insensitivity to rapamycin on any concentration of rapamycin media whereas BP12–2 and BP12–5 plants were not obviously inhibited by rapamycin until the concentration was reduced to 1nM as indicated in Figure A4. (B) FK506 does not inhibit the growth of WT and BP12 plants containing the exogenous yeast FKBP12. (C–F) The primary root length (C), lateral root number (D), lateral root length, (E) and root hair length (F) of 25 DAG WT and BP12 plants grown on rapamycin (10nM) or FK506 (20μM) medium were measured, respectively, indicating that rapamycin can specifically inhibit BP12 plants, but FK506 is unable to suppress plant growth in the absence or presence of exogenous yeast FKBP12. Error bars indicate SD for triplicates (n = 20).
