PHANTASTICA regulates leaf polarity and petiole identity in Medicago truncatula

Figures & data

Table 1. Primer sequences

Name	Sequence	Source
MtYAB5 QRT-F	TGAATCTCTT CCTTGATGAA CTG	This study
MtYAB5 QRT-R	CTGTCATGGC AAGATGTTCA GG	This study
MtKAN2 QRT-F	GTCTCACTTA CAGATGTATC GG	This study
MtKAN2 QRT-R	GGAAGAGTTG CTCCAAAGAC CA	This study
MtPIN10 QRT-F	CCATGTCTTT AGAGGTGGTG GTG	Peng and Chen, 2011
MtPIN10 QRT-R	CACTGGTCCT TCTTTGTCCA CAC	Peng and Chen, 2011
MtACTIN QRT-F	GCAGATGCTG AGGATATTCA ACC	Ge et al., 2014
MtACTIN QRT-R	CTTCGTCACC AACATAGGCA TCC	Ge et al., 2014

Figure 1. Quantitative RT-PCR analysis of leaf polarity and patterning gene expression in Medicago truncatula. (A) KAN2, (B) YAB5, and (C) MtPIN10 gene expression was analyzed in shoot buds and young leaves of one-month-old wild-type (R108) and mtphan mutant. The expression level was normalized using a Medicago ACTIN gene. Shown are means ± s.d. (n = 3).

Figure 2. Models of petiole identity regulation in Medicago truncatula. MtPHAN, likely with the AS2 ortholog, represses expression of ELP1, preventing ectopic acquisition of motor organ identity in petioles of wild-type plants. In the absence of MtPHAN, ELP1 is ectopically expressed to promote motor organ identity in petioles of mature mtphan mutant. Red and green colors indicate active and inactive gene expression, respectively.