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Addendum

Rhamnogalacturonan-II cross-linking of plant pectins via boron bridges occurs during polysaccharide synthesis and/or secretion

, &
Article: e28169 | Received 29 Jan 2014, Accepted 10 Feb 2014, Published online: 06 Mar 2014

Figures & data

Figure 1. Boron-bridging of RG-II and separation by gel electrophoresis. (A) Schematic representation of boron-bridging of two pectin molecules (B) Gel electrophoresis separation of monomeric and dimeric RG-II. Sample 3 shows separation of oligomers with DP ~6–16 (hexasaccharide to hexadecasaccharide) resulting from the incomplete digestion of homogalacturonan.

Figure 1. Boron-bridging of RG-II and separation by gel electrophoresis. (A) Schematic representation of boron-bridging of two pectin molecules (B) Gel electrophoresis separation of monomeric and dimeric RG-II. Sample 3 shows separation of oligomers with DP ~6–16 (hexasaccharide to hexadecasaccharide) resulting from the incomplete digestion of homogalacturonan.