Figures & data
Figure 1. Osmotic stress tolerance in Chimeric REpressor gene Silencing Technology (CRES-T) lines. Germination rates (A, B, n = 10 × 3 sets), root length (C, D, n = 10), rate of seedlings with visible cotyledons (E, n = 10 × 3 sets), and appearance of seedlings (F) of wild-type (WT) and CRES-T lines (ADA2b-SRDX, Msantd-SRDX, DDF1-SRDX, DREB26-SRDX, AtGeBP-SRDX, and ATHB23-SRDX) were monitored on medium supplemented with 500 mM (A, C, E, F) and 600 mM (B, D, E) mannitol. A seed was regarded as germinated when the radical protruded through the seed coat. Cotyledons were regarded as visible when they appeared from the seed coat. Photographs were taken at 13 d after sowing. Only a representative photo is shown. Error bars represent standard errors. Scale = 1 cm. * and ** denote significant difference from WT at P < 0.05 and 0.01, respectively. ND, not detected.
Figure 2. Proline content and delta 1-pyrroline-5-carboxylate synthase (P5CS) and proline dehydrogenase (PDH) gene expression in Chimeric REpressor gene Silencing Technology (CRES-T) lines. Proline contents (A) and expression of P5CS (B) and PDH (C) genes in wild type (WT) and CRES-T lines under 600 mM mannitol stress were determined. Error bars represent standard errors. *Significant difference from WT at p < 0.05.
