The plasticity of priming phenomenon activates not only common metabolomic fingerprint but also specific responses against P. cucumerina

Figures & data

Figure 1. Non-supervised PCA representation of signals obtained from a non targeted analysis by HPLC-QTOFMS to monitor metabolomic changes during fungal invasion. (A) PCA1 vs PCA2 and (B) PCA1 vs PCA3. Three week old seedlings were sprayed inoculated with 10E3 spores.ml⁻¹ of P. cucumerina and samples for analysis were collected 48hpi. Leaf material from 15 individual plants were pooled together for each treatment x genotype combination. Data points represent two technical replicates from three independent experiments injected randomly into the HPLC-QTOFMS. The signals corresponding to different treatments were compared using the non-parametric Kruskal-Wallis test, and only data with a P value lower than 0.01 between groups was used for subsequent processing.

Figure 2. (A) Heat map analysis of the signals extracted from the Kruskal-Wallis test with a P value lower than 0.01 between groups performed with Marvis (Filter and Cluster packages) and (B) Venn diagrams of detected primed signals.

Table 1. Tentatively identified pathways

Pathways involved in lin1 defense priming	Nº of primed metabolites	Pathways involved in BABA defense priming	Nº of primed metabolites
Metabolites derived from shikimate pathway	25	Metabolites derived from shikimate pathway	30
Biosynthesis of phenylpropanoids	25	Biosynthesis of phenylpropanoids	26
Tryptophan metabolism	23	Tryptophan metabolism	20
Glucosinolate biosynthesis	22	Glucosinolate biosynthesis	16
Arginine and proline metabolism	15	Arginine and proline metabolism	26
Biosynthesis Type II poliyketide products	22	Purine metabolism	20
Lignans	15	Tyrosine metabolism	16
Flavonoid Biosynthesis	11	Polycyclic aromatic hydrocarbon degradation	22

Detected signals by HPLC-QTOF MS with strong priming behavior extracted from Venn Diagram were assigned to different metabolic pathways. Signals were assigned by using Metlin database and pathways identified by using KEGG and AraCyc databases.