Fine-tuning of early events in the jasmonate response

Figures & data

Figure 1 Identification of transcriptional regulators of JAZ1/TIFY10a expression. (A) Regulation of JAZ1/TIFY10a expression by transient expression of MYC2 and ORA47 open reading frames (ORF). Tobacco (Nicotiana tabacum) protoplasts were transfected with a PJAZ1:fLUC reporter construct, a P35S:ORF effector construct, and a P35S:rLUC normalization construct. The PJAZ1 construct covered a 1356-bp region preceding the start codon. Averaged (n ≥ 8) normalized fLUC activities are plotted relative to the P35S:GUS control. Error bars represent standard error. Asterisks indicate significant effects (t-test, p-value <0.05). (B) Yeast one-hybrid interaction with the JAZ1/TIFY10a promoter. Full-length ORFs fused to GAL4AD were expressed in a PJAZ1:HIS3 reporter strain. The empty Gateway destination vector was used as a negative control. Yeast was grown for 3 days on selective medium in presence of 3-AT. All methods are as described.5

Pauwels L, Morreel K, De Witte E, Lammertyn F, Van Montagu M, Boerjan W, Inzé D, Goossens A. Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells. Proc Natl Acad Sci USA 2008; 105:1380 - 1385

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