Antisense oligodeoxynucleotide inhibition as a potent diagnostic tool for gene function in plant biology

Figures & data

Figure 1 Zymogram of starch branching enzyme (SBE) activities. Barley leaves were incubated in sorbitol or sucrose with sense or antisense SBE ODNs followed by SBE zymogram analysis.17 Antisense and corresponding sense ODNs10 were constructed as follows. SBEI (NCBI Accession number AY304541), nt 15–32; SBEIIA (NCBI Accession number AF064560), nt 1–18; SBEIIB (NCBI Accession number AF064561, nt 116–133.

Figure 2 ODNs “piggyback” on transported sugar molecules. A pictorial representation of ODNs utilizing the sucrose translocator (SUT) to enter plant cells. The confocal microscope projection image shows uptake of fluorescently labeled ODNs (green) in barley leaves after 24 h incubation in 200 mM sucrose.10 Autofluorescence from chloroplasts is shown in red. The superimposed cartoon shows the coupled symport of H⁺ (white dot), sucrose (blue) and ODN (green) through the SUT (red). Scale bar, 10 µm.

Borén M, Glaring MA, Ghebremedhin H, Olsson H, Blennow A, Jansson C. Molecular and physicochemical characterization of the high-amylose barley mutant Amo1. J Cereal Sci 2007; In press

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Sun C, Höglund AS, Olsson H, Mangelsen E, Jansson C. Antisense oligodeoxynucleotide inhibition as a potent strategy in plant biology: Identification of SUSIBA2 as a transcriptional activator in plant sugar signaling. Plant J 2005; 44:128 - 138

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