Figures & data
Figure 1 Visualization of stroma-targeted DsRed and chlorophyll autofluorescence in living mesophyll cells of wild-type plants by laser-scanning confocal microscopy. A excised control leaf (A, Light) and an individually darkened leaf (B, IDL) from plants grown under 14 h-photoperiod condition and a leaf from whole-plant darkened condition (WD, C) for 5days were incubated with 1 µM concanamycin A in 10 mM MES-NaOH (pH 5.5) at 23C° for 20 h in darkness. Stroma-targeted DsRed appears green and chlorophyll fluorescence appears red. In merged images, overlap of DsRed and chlorophyll fluorescence appears yellow. Small vesicles with stromal-targeted DsRed, i.e. RCBs, can be found in the vacuole (A, B). In IDL (B), massive accumulation of stroma-targeted DsRed is entirely seen in the vacuolar lumen and chloroplasts losing DsRed fluorescence are found in some cells. Bars = 50 µm.
