Prolonged root hypoxia effects on ethylene biosynthesis and perception in tomato fruit

Figures & data

Figure 1 Ethylene content in fruits of control (▴) and hypoxically treated (■) plants during the maturation phase. Values represent the mean ± SD of at least three measurements. MG, mature green; B, breaker; T, turning; O, orange; RR , red ripe.

Figure 2 Semi-quantitative RT-PCR analysis of genes implicated in ethylene biosynthesis in fruits of control and hypoxically treated plants during the maturation phase. Signals were acquired using the Biorad Gel doc system and quantified with the quantity one software (Biorad). Values represent the mean ± SD (from two independent experiments) of the relative intensity of each signal normalized to MG control and EF1α signals. ACS, 1-aminocyclopropane-1-carboxylic acid synthase; ACO, 1-aminocyclopropane-1-carboxylic acid oxidase.

Figure 3 Semi-quantitative RT-PCR analysis of genes implicated in ethylene perception in fruits of control and hypoxically treated plants during the maturation phase. Signals were acquired using the Biorad Gel doc system and quantified with the quantity one software (Biorad). Values represent the mean ± SD (from two independent experiments) of the relative intensity of each signal normalized to MG control and EF1α signals. ETR: ethylene receptor.

Table 1 Primers used for semi quantitative RT-PCR analysis

Gene name	Primer name	Sequence	Size (bp)	Accession number
EF1α	Direct	ATG TTG GGT TCA ATG TTA AG	479	X14449
	Reverse	ATC ACA CTG CAC AGT TCA C
Acs2	Direct	TTG TGA CGA AAT CTA CGC	752	X62536
	Reverse	AAC GAA CTA ATG GTG AGG
Acs4	Direct	TGT CAC TTT GGA AAC TCA TC	317	X59146
	Reverse	ACT ACG AGC AAG GAA TTG G
Acs6	Direct	TAC TCG TAC AAT GAT GCC	634	AB013100
	Reverse	AAC GAT GTG TGT AGA AGC
Aco4	Direct	TGT ATG AAG TTC CTG ACC	487	AB013101
	Reverse	TCT GGT GCT GGA AAT ATG
ETR1	Direct	TGT TAC GAA GTT GCT GTA G	420	U41103
	Reverse	GGA ATA TGA ACA CTT CTG G
ETR3	Direct	ATA TAC CAC TAG TAT TCA CC	452	U38666
	Reverse	GAA TTA GGT TAG TTA CAA GAC
ETR4	Direct	GTA GAA TGA TGT AAT TGA TG	359	AF118843
	Reverse	GAA CAC TTA GAA ATG GAG C
ETR5	Direct	GTA CTC ATC TTG GAT CTT C	521	AF118844
	Reverse	CTA ACA TTC TTC TGT TTC TG

Size of the PCR product is indicated in base pair as well as the accession number of the corresponding cDNA clones. EF1α, elongation factor; ACS, 1-aminocyclopropane-1-carboxylic acid synthase; ACO, 1-aminocyclopropane-1-carboxylic acid oxidase; ETR, ethylene receptor.