Figures & data
Figure 1 Alignment of amino acid sequence of Lsi2-like Si efflux transporters in rice (OsLsi2) and pumpkin. Two copies of Lsi2-like gene encoding CmLsi2-1 and CmLsi2-2 were obtained from pumpkin cultivars; Shintosa (B+) and Super-unryu (B−).
Figure 2 Phylogenetic tree of Si efflux transporter gene homologs in pumpkin (Cm−), rice (Os−), maize (Zm−), barley (Hv−) and Arabidopsis (At−). The 0.1 scale shows substitution distance.
Figure 3 Efflux transport activity for Si in Xenopus oocyte. Oocytes expressing CmLsi2-1, CmLsi2-2 or no expression control (water injected) were injected with Si labeled with 68Ge. The radioactivity released from the oocytes was determined. Data are means ± SD of three biological replicates. * means significant difference at p < 0.05 (by student's t test).
Figure 4 Tissue-specific expression of CmLsi2-1 (A) and CmLsi2-2 (B) in the roots and leaves of two pumpkin cultivars: Shintosa (B+, bloom rootstock) and Super-unryu (B−, bloomless rootstock). The expression level was determined by absolute quantitative RT-PCR. Data are means ± SD of three biological replicates. Different letters above the columns indicate statistically significant differences at p < 0.05 by Tukey's test.
