Figures & data
Figure 1. Schematic representation of organoid generation from rectal suction biopsies. Crypts are isolated from rectal biopsies of CF subjects that self-organize into closed 3D multicellular structures consisting of a single epithelial layer and an internal lumen termed organoids upon in vitro culture.
Figure 2. CFTR function measurements using intestinal organoids. (A) Schematic representation of the method to measure forskolin-induced organoid swelling. (B) Confocal images of calcein-green labeled F508del/F508del organoids with or without VX-809 + VX-770 treatment at the indicated time points of forskolin stimulation.
