Figures & data
Figure 1 Cloning of the porcine Tex11 from adult pig testis. A partial sequence (1,028 bp) of the porcine Tex11 (slightly over 1 Kb) was amplified from adult pig testis by RT-PCR and resolved on a 1.2% agarose gel. Lane a: DNA ladder. Lane b: Tex11. Lane c: Gapdh (983 bp).
Figure 2 Multiple alignments of the partial porcine Tex11 sequence with the corresponding mouse and human Tex11 sequences.
Figure 3 The expression profile of Tex11 in pig tissues. The porcine Tex11 is specifically expressed in the adult pig testis. It is not detected in somatic tissues examined in this study. The size of the PCR product for porcine Tex11 and Gapdh is 1,028 bp and 983 bp, respectively.
Figure 4 TEX11 protein is detected in the mature pig testis. TEX11 protein was only detected in the adult pig testis where complete spermatogenesis occurs. In the 10 week old testis, the only germ cells present were spermatogonia and TEX11 was undetectable. The estimated size of porcine TEX11 is slightly over 100 kD, which is similar to the size of homologs reported in mice and humans.
Figure 5 The cell-type specific expression of TEX11 in the porcine testis. TEX11 is highly expressed in spermatocytes in mature (>12 months of age) porcine testis (a). The expression pattern is very similar to that of the mouse TEX11 in mature mouse testis (b). Spermatocytes are labelled with white arrows. Stages of spermatogenesis are indicated with Roman numerals. Scale bar: 10 µm.
