Quantitative and conformational characterization of lysozyme deposited on balafilcon and etafilcon contact lens materials

Abstract

Purpose. To determine whether differences in lysozyme deposition and/or activity exist on worn etafilcon and balafilcon contact lenses following care with a polyquaternium-based system (PQ) or a polyhexanide-based system (PHMB). Methods. Following acid-based deposit extraction, lysozyme concentration was determined via Western blotting and lysozyme activity was determined by a micrococcyl assay. Results. Lysozyme deposition on etafilcon lenses was greater following disinfection with the PHMB-based system (1551 ± 371µg/lens vs 935 ± 271µg/lens; p < 0.001). Deposition on balafilcon lenses was not influenced by the care regimen (10 ± 3.5µg/lens vs 10 ± 5µg/lens; p = 0.89). For both materials, the percentage of denatured lysozyme was greater when they were exposed to the PHMB-based system (28 vs 21%; p = 0.05 (etafilcon) and 57 vs 40%; p = 0.04 (balafilcon)). Conclusions. The quantity and conformation of lysozyme deposited on hydrogel contact lens materials is significantly influenced by both lens material and care regimen.