Association of cytokines polymorphisms with chronic peridontitis and rheumatoid arthritis in a Mexican population

Abstract

Objective: Historically, it has been shown that rheumatoid arthritis (RA) and periodontitis (PE) share pathophysiological similarities and possibly a genetic background. In order to elucidate the genetic background between both diseases, we evaluated the distributions of five SNPs genotypes and all the possible haplotypes composed in subjects with isolated RA, PE, combined diseases and healthy controls.

Materials and methods: The study population consisted of 280 Mexican subjects. Genomic DNA was isolated from buccal epithelial cells collected by cheek scrapings and analyzed for the determination of the following SNPs: IL-1α + 4845 (rs17561), IL-1α −889 (rs1800587), IL-1β + 3954 (rs1143634), IL-1β −511(rs16944) and TNF-α −308 (rs1800629).

Results: After adjustment for age, sex and smoking status, multiple logistic regression analysis revealed a no significant association in the genotype frequencies of TNF-α −308 and IL-1α + 4845 SNPs. Otherwise a significant association was observed in IL-1β + 3954 and IL-1β −511 (p < 0.05) while IL-1α −889 was of borderline statistical significance (p = 0.054). Also, we found three negative associated haplotypes with PE: IL-1α + 4845 G/IL-1β −511 A, IL-1β + 3954 C/IL-1β −511 A and interestingly IL-1α −889 C/IL-1β −511 A also with a positive association with RA.

Conclusions: Some genotypes and haplotypes are associated with the diseases. But it seems that the genetic background of the association between RA and PE needs to be explored deeper.

Keywords:

• Cytokine SNPs
• genetics
• haplotypes

Acknowledgements

This investigation was supported by CONACYT CB-2014-01 [grant No. 242939], CONACYT INFR-2014-01 [grant No. 226467).

Disclosure statement

There is no conflict of interest to declare.

Note on contributors

Rubén Abraham Domínguez-Pérez, Dental Surgeon, Specialty in Endodontics, PhD Student, Doctorado en Ciencias Biomédicas Basicas, Universidad Autonoma de San Luis Potosi.

Juan Pablo Loyola-Rodriguez, Dental Surgeon, Specialty in Pediatric Dentistry and Doctor in Dental Sciences, Professor of Dental School of Universidad Autonoma de San Luis Potosi, The Master’s Degree in Dental Science with Specialization in Advanced General Dentistry and National Researcher.

Carlos Abud-Mendoza, Medical Doctor, Specialty in Internal Medicine and Rheumatology, Director of the Regional Unit of Rheumathology and Osteoporosis at Central Hospital ?Dr. Ignacio Morones Prieto?. National Researcher.

Angel Gabriel Alpuche-Solis, Biologist, Doctor in Sciences. Professor and Researcher, Director of Laboratory of Molecular Biology. Instituto Potosino de Investigacion Cientifica y Tecnologica.

José Luis Ayala-Herrera, Dental Surgeon, Master in Dental Sciences, Universidad Autonoma de San Luis Potosi, PhD Student, Doctorado en Ciencias Biomédicas Basicas, Universidad Autonoma de San Luis Potosi.

Rita Elizabeth Martinez-Martinez, Dental Surgeon, Master in Dental Sciences, Doctor in Basic Biomedical Sciences. Professor of Dental School of Universidad Autonoma de San Luis Potosi, The Master?s Degree in Dental Science with Specialization in Advanced General Dentistry and National Researcher.

Additional information

Funding

This investigation was supported by CONACYT CB-2014-01 [grant No. 242939], CONACYT INFR-2014-01 [grant No. 226467).