Genetic polymorphisms in interleukin-6 and interleukin-1-beta were associated with dental caries and gingivitis

Abstract

Objective

Evaluate the association between single nucleotide polymorphisms (SNPs) in Interleukin-6 (IL-6) gene (rs1800795) and in Interleukin-1-beta (IL-1β) gene (rs1143627 and rs1143629) with dental caries and gingivitis in Brazilian children.

Material and methods

Three hundred and fifty-three children aged 8–11 years were included. Visible biofilm and gingival bleeding were evaluated by Community Periodontal Index. The International System for Detection and Assessment of Carious Lesions (ICDAS) was used to investigate dental caries. Real-time PCR evaluated SNPs in the DNA. Chi-square test, haplotype analysis and logistic regression were applied (alpha of 5%).

Results

The GG genotype in rs1800795 (IL-6) decreases the risk of gingivitis in a co-dominant model (p = .05; OR = 0.64). The GG genotype in rs1143627 (IL-1β) reduces the risk of dental caries (Co-dominant model: ICDAS₀ versus ICDAS_1-6: p = .05; OR = 0.55. ICDAS_0-2 versus ICDAS_3-6: p = .02; OR = 0.49. Recessive model: ICDAS₀ versus ICDAS_1-6: p = .005; OR = 0.48. ICDAS_0-2 versus ICDAS_3-6: p = .004; OR = 0.45. Logistic regression: ICDAS_0-2 versus ICDAS_3-6: p = .05; OR = 0.24; CI 95%= 0.05–1.00). The GG genotype in rs1143629 was more frequent in ICDAS₀ (p = .05; OR: 0.60). In the haplotype analysis, IL-1β was associated with gingivitis.

Conclusion

The rs1800795 in IL-6 gene was associated with gingivitis. The rs1143627 and rs1143629 in IL-1β were associated with dental caries and gingivitis.

Keywords:

• Dental caries
• gingivitis
• interleukin 6
• interleukin 1 beta
• genetic polymorphisms

Author Contributions

E.C.K, D.C.L and D.S.B.O designed the epidemiological study and coordinate sample collection. M.C.F.B, B.M.S.M.M. and C.L.B.R performed the data collection. M.C.F.B performed the DNA extraction. F.B.F e J.A B funding support e laboratory experiments design and coordination. C.L.B.R and S.S.P.B performed PCR laboratorial analysis. E.C.K., D.S.B.O and C.L.B.R analyzed and interpreted the data and wrote the article. All authors contributed to the final version of the article.

Disclosure statement

The authors declared that they have no conflicts of interest to this work.

Additional information

Funding

The Positivo University (Curitiba, Paraná, Brazil) and Minas Gerais State Research Foundation (FAPEMIG) supported this work.