A novel method for determining the relation between nasal polyposis and oxidative stress: the thiol/disulphide homeostasis

Abstract

Conclusion: It is thought that oxidative stress may be the major cause of the increase in the oxide thiol form in the study group. The relationship between oxidative stress status and dynamic thiol/disulphide in nasal polyposis now needs to be investigated.

Objectives: The purpose of this study was to evaluate the relation between nasal polyposis and thiol/disulphide homeostasis, used as a marker of oxidative stress, by measuring that exchange using a novel technique.

Materials and methods: The study group consisted of 40 patients (mean age = 46.75 ± 13.92 years) with bilateral nasal polyposis patients admitted to the hospital. The control group consisted of 31 (mean age = 43.20 ± 5.68 years) age, sex, and body mass index matched healthy subjects. Thiol/disulphide homeostasis concentrations were measured using a newly-developed method (Erel & Neselioglu).

Results: Native thiol and total thiol levels were lower in the study group compared to the control group (native thiol = 415.8 ± 69.1 μmol/L vs 448.7 ± 37.5 μmol/L, p < 0.05; total thiol = 449.02 ± 72.0 μmol/L vs 477.28 ± 44.5 μmol/L, p < 0.05, respectively). Disulphide level and the disulphide/native thiol and disulphide/total thiol ratios were higher in the study group compared to the control group (disulphide = 16.58 ± 5.04 μmol/L vs 14.28 ± 5.3 μmol/L, p < 0.05; disulphide/native thiol ratio = 4.07 ± 1.52% vs 3.14 ± 1.04%, p < 0.05, disulphide/total thiol ratio = 3.73 ± 1.23% vs 2.94 ± 0.92%, p < 0.05, respectively).

Keywords:

• Disulphide
• nasal polyposis
• oxidative stress
• thiol

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

The authors declare that they have no conflict of interest. Informed consent was obtained from all individual participants included in the study.