Prevalence of Fusobacterium necrophorum in tonsils from patients with chronic tonsillitis

Abstract

Conclusion: There was a high prevalence of Fusobacterium necrophorum (FN) in patients with chronic tonsillitis in the age group 15–23 years. This indicates that FN might play an important role in the pathogenesis of chronic tonsillitis in this age group, which is also the age group in which chronic or recurrent tonsillitis is most common.

Objectives: The role of FN in patients with acute and chronic tonsillitis is unclear. Thus, this study investigated the occurrence of FN in tonsils of patients with chronic tonsillitis. The aim of the study was to determine the prevalence of FN in patients that underwent tonsillectomy due to chronic tonsillitis. This study also investigated if FN was found at different areas in the tonsils.

Method: One hundred and twenty-six consecutive patients undergoing tonsillectomy due to chronic tonsillitis were included from the ENT clinics at Sunderby Hospital and Gällivare Hospital, Sweden. Both children and adults were included to encompass various age groups (age =2–57 years). Culture swabs were taken from three different levels of the tonsils – the surface, the crypts, and the inner core of the tonsils. Selective agar plates for detecting FN were used for culture. Culture was also made for detecting β-hemolytic streptococci, Haemophilus influenzae, and Arcanobacterium.

Results: FN was the most common pathogen (19%). The highest prevalence of FN was found in the age group 15–23 years (in 34% of the patients). FN was detected both at the surface and in the core of the tonsils. Furthermore, in the few patients where FN was not detected in all three areas, FN was always detected at the tonsillar surface, in spite of being an anaerobic bacterium. Streptococci group G and C also occurred most frequently (30%) in the same age group as FN (15–23 years), whereas Streptococci group A was more evenly spread among the age groups.

Keywords:

• Tonsillectomy
• human tonsils
• bacterial culture
• streptococci

Acknowledgments

We especially thank Mats Sellin, Umeå, and Peter Cettner, Sunderby Hospital, for their co-operation regarding the FN culture method and Robert Lundqvist for assistance with the statistical analysis.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Funding

The present study was financed by Umeå University, Akademisk miljö, Sunderby Hospital and the Research Unit at the County of Norrbotten.