Gastric-type H⁺,K⁺-ATPase in mouse vestibular end organs

Abstract

Conclusion: Gastric type H⁺,K⁺-ATPase in the vestibular end organs may be of importance for K⁺ circulation and may also be related to pH regulation in vestibular end organs and endolymphatic sac.

Objective: To analyze the expression of gastric-type H⁺,K⁺-ATPase in normal mouse vestibular end organs.

Methods: 8 weeks old CBA/J mice were used in this study. The presence of gastric-type H⁺,K⁺-ATPase α and β in the vestibular end organs, viz. utricle, saccule, ampulla, vestibular ganglion, and endolymphatic sac, was investigated using immunohistochemistry.

Results: In the vestibular end organs, H⁺,K⁺-ATPase α and β were almost identical. H⁺,K⁺-ATPase was expressed in sensory cells, the basolateral surface of dark cells, fibrocytes, in vestibular ganglion cells, and in the upper region of the endolymphatic sac epithelial cells.

Keywords:

• Hydrogen, potassium-adenosine triphosphatase
• vestibular end organ
• immunohistochemistry

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Funding

This study was supported by a Grant-in-Aid for Scientific Research (25462643) provided by the Ministry of Education, Science and Culture, Japan, and also by the Swedish Medical Research Council (Grant No. 17X-7305).