The effect of platelet-rich fibrin membrane on the repair of perforated tympanic membrane: an experimental study

Abstract

Conclusion: Platelet-rich fibrin (PRF) membrane could be used successfully in the repair of tympanic membrane perforation and wound healing.

Objectives: To evaluate the effect of platelet-rich fibrin membrane in the repair of perforated tympanic membrane.

Methods: After otoscopic examination, a 3-mm perforation was made in the posterior quadrant of both tympanic membranes of 50 adult male Sprague-Dawley rats. Venous blood was withdrawn from the rats, then centrifuged. PRF was obtained in membrane form. The membrane was placed on the right tympanic membrane perforation. The perforations on the left side were left to spontaneously heal and, thus, formed the control group. Daily examinations were made of 20 rats and the time to healing of the tympanic membrane was recorded. The remaining 30 rats were separated into five groups of six, and histopathological examination was made. Evaluation was made in respect of the presence of oedema in the lamina propria, neovascularization, fibroblastic reaction, and inflammatory cells.

Results: The healing time of the tympanic membrane perforation was determined as mean 10.3 ± 2.18 days in the study group applied with PRF and 17 ± 2.40 days in the control group. Higher values in respect of fibrosis and neovascularization were obtained in the study group.

Chinese abstract

结论 富血小板血纤维蛋白 (PRF) 膜可以成功用于修复鼓膜穿孔和伤口愈合。

目的 评价富血小板血纤维蛋白膜在穿孔鼓膜修复中的作用。

方法 在耳镜检查之后, 在50个成年雄性Sprague-Dawley大鼠的两个鼓膜的后部区域进行3毫米穿孔。从大鼠体内抽取静脉血, 然后离心。获得薄膜形式的PRF。将薄膜置于右鼓膜穿孔上。让左侧穿孔自行愈合, 从而形成对照组。每日检查20只大鼠, 记录鼓膜愈合时间。将剩余的30只大鼠分成5组, 每组6只, 进行组织病理学检查。就固有层中水肿的存在、新血管形成、成纤维细胞反应和炎症细胞进行评价。

结果 应用PRF的研究组的鼓膜穿孔愈合时间被确定为平均10.3 ± 2.18天, 在对照组中为17 ± 2.40天。在研究组中取得了更高的纤维化和新血管形成值。

Keywords:

• Wound healing
• tympanic membrane perforation
• fibrosis
• platelet-rich fibrin membrane
• inflammation

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.