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Abstract
Objective: In the present study, we aimed to clarify the expression of thymic stromal lymphopoietin (TSLP), a key trigger of Th2-type allergic disease, in the middle ear mucosa of eosinophilic otitis media (EOM).
Methods: An immunohistological study of TSLP was conducted in patients with EOM and in animal models of EOM constructed by intraperitoneal and intratympanic injection of ovalbumin for 7 and 14 days. In addition, the messenger RNA (mRNA) expression of TSLP in the middle ear mucosa of the animal models was analyzed using real-time PCR, and was compared with that of the control animals.
Results: Immunoreactivities for TSLP were observed in the middle ear mucosa around the tympanic ostium of the eustachian tube of patients with EOM. In the animal model, strong immunoreactivity for TSLP was also observed in the eustachian tube epithelium, and mRNA expression of TSLP in the seven-day stimulated animals was significantly higher than that in the controls.
Conclusion: The present study indicates that the presence of epithelium-derived TSLP in the eustachian tubes plays an important role in the onset of EOM.
Chinese abstract
目的：本研究旨在阐明嗜酸细胞性中耳炎（EOM）中耳粘膜中胸腺基质淋巴细胞生成素（TSLP） 的表达。该生成素是Th2型过敏性疾病的一种关键触发因子。
方法：对EOM患者和EOM动物模型进行了TSLP免疫组织化学研究, 对腹腔和鼓室内注射卵清蛋白7天和14天。另外, 使用实时PCR分析动物中耳粘膜中的TSLP的信使RNA（mRNA）表达, 并将其与对照动物进行比较。
结果：EOM患者咽鼓管鼓膜周围中耳粘膜中可观察到TSLP免疫反应。在动物咽鼓管上皮中也观察到TSLP的强免疫反应, 并且经七天刺激的动物的TSLP的mRNA表达明显高于对照组。
结论：本研究表明, 源于上皮的TSLP在咽鼓管内的存在对于EOM发病起着重要作用。
Acknowledgments
We would like to thank Professor A. Kurose for his helpful comments.
Disclosure statement
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.