Solute carrier family 4 member 1 might participate in the pathogenesis of Meniere's disease in a murine endolymphatic hydrop model

Abstract

Background: To date, the pathogenesis of Meniere’s disease (MD) remains unclear. Previous research found that the SLC4A1 gene significantly down-regulated.

Aims: This study sought to understand the effect of SLC4A1 on the pathogenesis of MD. ELH C57 mice models were induced by intraperitoneal injection of AVP.

Material and methods: The mRNA expression levels of SLC4A1, SLC4A10 and SLC26A4 were monitored by real-time quantitative PCR, the protein expression levels of SLC4A1 were monitored by immunoblotting and immunofluorescence before and after the ELH. DIDS is an inhibitor of SLC4A1. The expression levels of SLC4A1 were also monitored in the AVP + DIDS group.

Results: We successfully established the model of ELH after applied AVP. The results of HE staining showed displacement of Reissner's membrane with bulge to scala vestibule in ears of the AVP group. Cochlea/ELS SLC4A1 protein and SLC4A1, SLC4A10, SLC26A4 mRNA expressions were reduced significantly in C57 mice of the AVP group. The SLC4A1 protein expression levels and SLC4A1, SLC4A10, SLC26A4 mRNA expression levels declined more obvious in the cochlea and ELS in C57 mice of the AVP + DIDS group.

Conclusions and significance: SLC4A1 was a protective factor in the pathogenesis of MD, but the mechanisms were unknown.

背景：迄今为止, 梅尼埃病（MD）的发病机制尚不清楚。先前的研究发现SLC4A1基因显著下调。

目的：探讨SLC4A1对MD 发病机制的影响。ELHC57小鼠模型由 腹腔注射AVP所诱导。

材料与方法：采用实时定量PCR法检测SLC4A1,、SLC4A10 和 SLC26A4 的mRNA表达水平。免疫印迹法和免疫荧光法检测ELH前后SLC4A1蛋白表达水平。DIDS是SLC4A1的抑制剂。同时, 在AVP + DIDS组检测SLC4A1的表达水平。

结果：成功地建立了应用AVP后的ELH模型。HE染色结果显示AVP组耳内Reissner膜移位以及前庭鳞状突起。AVP组C57小鼠耳蜗/ELS SLC4A1蛋白及SLC4A1,、SLC4A10 和 SLC26A4 的mRNA表达水平显著降低。AVP + DIDS组C57小鼠耳蜗和耳蜗中SLC4A1蛋白表达水平和SLC4A1,、SLC4A10 和 SLC26A4 的mRNA表达水平下降更为明显。

结论及意义：SLC4A1是MD发病的保护因子, 但其机制尚不清楚。

Keywords:

• SLC4A1
• SLC4A10
• SLC26A4
• DIDS
• Meniere’s disease
• endolymphatic hydrops

Acknowledgments

The authors thank all participants in this study.

Disclosure statement

The authors declare that they have no competing interests.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China [No. 81670932], Natural Science Foundation of Shandong Province [ZR2016QZ007], Key Technology Research and Development Program of Shandong [2017CXGC1213], the Focus on research and development plan in Shandong province [2018GSF121027].