Multifrequency Tympanometry and Histopathology in Chinchillas with Experimentally Produced Middle Ear Pathologies

Abstract

Middle ear lesions were produced in chinchillas by introducing fascia, Gelfoam?, silastic or a combination of these materials into the labyrinthine and mastoid bullae. After 1-2 months, conventional 226 Hz and multifrequency tympanometry (MFT) were performed and the animals were sacrificed for histopathological analysis of the middle ear. Specific middle ear lesions were created including abnormal tympanic membrane thickness, tympanic membrane mass, tympanic membrane adhesion, mastoid bulla obstruction, ossicular adhesions and reduced middle ear volume. Several potentially strong associations emerged between tympanometric measures and pathological conditions. The strongest were the combinations of thick tympanic membrane and irregular multifrequency tympanograms, tympanic membrane mass and notched 226 Hz admittance tympanograms, tympanic membrane adhesions and low 226 Hz admittance, and tympanic membrane adhesions and low resonant frequency. 226 Hz static admittance had a sensitivity of 0.73 and a specificity of 0.75 for detecting significant middle ear pathology. Tympanometric width was not an effective diagnostic test, separately or in combination with static admittance. The most effective test based on 226 Hz measures was the combination of low admittance or admittance notch. MFT was superior to 226 Hz tympanometry. The combination of low resonance or double resonance or irregular patterns had a sensitivity and specificity of 0.91 and 1.0, respectively. Combining 226 Hz tympanometry with MFT improved the performance relative to 226 Hz tympanometry alone but not relative to MFT alone. The results support clinical reports that multifrequency tympanometry detects some middle ear pathologies that are not detected by conventional 226 Hz tympanometry.