Characterization of Oligosaccharides from an Enzymatic Hydrolyzate of Red Yeast Cell Walls by Lytic Enzyme

Abstract

Eight oligosaccharides were isolated from an enzymatic hydrolyzate of cell walls of Rh.glutinis K–24 with red yeast cell wall lytic enzyme of Penicillium lilacinum. Two of these oligosaccharides were composed of mannose only and the others were composed of mannose and glucose. Linkage type between mannoses in the oligosaccharides was mainly β-1-4 bond. All of the linkages between glucose and mannose were β-1-6 bonds. The red yeast cell wall lytic enzyme hydrolyzed β-1-3 mannoside bond. Thus there might be a considerable amounts of β-1-3 mannoside bonds in cell walls. From these results, it might be concluded that main component of cell walls of red yeast was a β-glucomannan and the lytic enzyme from Pen. lilacinum was a glucomannase.