Structures of Integrated DNA Containing Human Adenovirus E1A Gene in Transgenic Mice

Abstract

One of the DNAs, Charon 10-gE1A-2, pSV2-gpt-gE1A, or Ad12 E1A, was microinjected separately into pronuclei of fertilized mouse eggs and fourteen transgenic mouse lines were obtained. The structures of integrated DNAs in these transgenic mice were analyzed by Southern blot hybridization. Head-to-head and tail-to-tail structures were found, although the injected molecules had a tendency to join each other in head-to-tail tandem forms when multiple copies were integrated. The analyses of the mice containing pSV2-gpt-gE1A showed that most of the restriction sites at the end of the injected molecules were restored at the tandem junctions. Furthermore, the integrated DNAs at the junctions to the host chromosomal DNA were deleted and/or rearranged.

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