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Abstract
Based on the reaction between acetylacetone-formaldehyde and β-lactamic antibiotics at pH=4.0, in which product can emit strong fluorescence, a selective simple fluorimetric method is described for the determination of both α-aminocephalosporins (namely cepharadine and cefalexin) and 6-aminopenicillanic acid (6-APA) in pure form and in pharmaceutical form. Other β-lactamic antibiotics free from α-amino group do not interfere with the assay. The linear ranges are 1.0×10−4-8.0×10−2 mg/ml, 2.0×10−4-3.0×10−2 mg/ml and 3.0×10−4-2.0×10−2mg/ml for cepharadine, cefalexin and 6-APA, respectively. Their detection limits (S/N=3) are 3.0×10−5mg/ml, 4.0×10−5mg/ml and 6.0×10−5mg/ml.