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ABSTRACT
A new flow injection method for the determination of riboflavin based on the inhibition of the intensity of chemiluminescence (CL) from the luminol-K3Fe(CN)6 system is described. While riboflavin mixed with K3Fe(CN)6, by the fast oxidation reaction between riboflavin and K3Fe(CN)6, K4Fe(CN)6 was generated, which then inhibited the CL reaction of K3Fe(CN)6 and luminol in alkaline aqueous solution. The CL emission was correlated with the riboflavin concentration in the range from 0.032 to 100 μg·ml−1, and the detection limit was 0.01 μg·ml−1 (3σ). A complete analysis could be performed in 2 min with a relative standard deviation of less than 2.2%. The influence of foreign species was studied and the method has been applied successfully to the determination of riboflavin in pharmaceutical samples, the recovery was from 98.0% to 102%.