Miniaturized Capillary Electrophoresis with Amperometric Detection: Fast Separation and Detection of Bioactive Amines

Abstract

A novel miniaturized analytical system for fast separation and detection of bioactive amines, based on the coupling of a micromachined capillary electrophoresis (CE) system with a Cu amperometric detector (CE‐AD) is described. Tryptamine, tryptophan, and tyramine could be detected down to the 1.5×10⁻⁶∼5.8×10⁻⁷ M level with linearity up to the 2.0×10⁻⁴ M level examined. The injection‐to‐injection reproducibility for analytes in peak current (RSD<3.6%) and for migration times (RSD<1.4%) were excellent. While the concept of miniaturized separation/detection systems is presented within the framework of food samples, such devices should be attractive for fields such as environment and clinic as an alternative to conventional CE and µ‐CE.

Keywords:

• Miniaturized capillary electrophoresis with amperometric detection
• bioactive amine
• tryptamine
• tyramine

The authors are grateful for the financial support provided by the National Science Foundation of China (grant No. 20375013) and the Ph.D. Program Scholarship Fund of ECNU.