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Analytical Letters Volume 39, 2006 - Issue 6
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Bioanalytical

Development and Optimization of an Indirect Enzyme‐Linked Immunosorbent Assay for Thiamphenicol

Kai Hao School of Food Science and Technology, Southern Yangtze University, WuXi City, Jiangsu, P.R. China
,
Shidong Guo School of Food Science and Technology, Southern Yangtze University, WuXi City, Jiangsu, P.R. ChinaCorrespondence[email protected]
&
Chuanlai Xu School of Food Science and Technology, Southern Yangtze University, WuXi City, Jiangsu, P.R. ChinaCorrespondence[email protected]
Pages 1087-1100 | Received 03 Dec 2005, Accepted 17 Jan 2006, Published online: 25 Oct 2011
 
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Abstract

The specificity of an enzyme‐linked immunosorbent assay (ELISA) was modified by changing the chemical structure of the haptenated coating antigen. A competitive indirect immunoassay was developed, evaluated, and validated to measure thiamphenicol (TAP) in eels. The working range of the assay was placed between 0.45 and 8.35 µg/L. Moreover, the influence of several physicochemical parameters, such as incubation time, ionic strength, detergent concentration, and pH, were studied. The specificity of this immunoassay was evaluated using 23 structurally related compounds. Finally, a good correlation was observed between the chromatographic and immunoassay techniques while analyzing five certified eel samples.

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