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Original Articles

Alcohol Biosensor Based on the Immobilization of Meldola Blue and Alcohol Dehydrogenase into a Carbon Nanotube Paste Electrode

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Pages 1643-1655 | Received 15 Dec 2005, Accepted 20 Feb 2006, Published online: 23 Aug 2007
 

Abstract

The preparation and electrochemical characterization of a new carbon nanotube paste (CNTP) amperometric biosensor for ethanol is described. The biosensor is formed by a CNTP electrode modified with the phenoxazine compound Meldola Blue (MB) as mediator. It shows a good electrocatalytic activity towards NADH oxidation at potentials around 0.0 V vs. Ag/AgCl, which represents a strong diminution in the overpotential. The biosensor is based on the activity of a commercially available alcohol dehydrogenase enzyme (ADH) and is realized by co‐immobilizing the enzyme ADH, the coenzyme NAD+, and the mediator into the paste.

Analytical parameters such as enzyme, co‐enzyme and mediator immobilization concentration, pH, temperature, and probe lifetime are studied and optimized.

The biosensor yields a linear response to 1×10−7 –4×10−6 mol/L ethanol at an operating potential of +50 mV (vs. Ag/AgCl), where interfering reactions do not occur, with a detection limit of 1×10−7 mol/L and a good reproducibility (RSD of 2.8%, n=6).

Application of the biosensor to the determination of ethanol in alcoholic beverages was achieved successfully.

The financial support of the Ministry of University and Scientific Research (MURST) is acknowledged.

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