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Analytical Letters Volume 40, 2007 - Issue 1
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Bioanalytical

Determination of Dibutyl o‐Phthalate by Antigen‐Coated Competitive Fluorescence Immunoassay

Ming‐Cui Zhang College of Environmental Science and Engineering, Donghua University, Shanghai, Wuhu, China; College of Chemistry and Materials Science, Anhui Normal University, Wuhu, China
,
Qiong‐E Wang College of Chemistry and Chemical Engineering, Donghua University, Shanghai, China
&
Hui‐Sheng Zhuang College of Environmental Science and Engineering, Donghua University, Shanghai, P. R. ChinaCorrespondence[email protected]
Pages 127-137 | Received 10 May 2006, Accepted 08 Jul 2006, Published online: 25 Feb 2007
 
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Abstract

A reliable and sensitive competitive fluorescence immunoassay for the quantitative determination of dibutyl phthalate (DBP) was developed. The hapten of dibutyl phthalate was synthesized. The hapten‐protein conjugate was used as an immunogen, demonstrating that it is suitable for polyclonal antibody production. With this polyclonal antibody, a novel fluorescence immunoassay for detection of DBP was described. Under best conditions, DBP can be determined in the concentration range of 0.1∼100 µg/L with a detection limit of 0.05 µg/L. The cross‐reactivities of the anti‐DBP antibody to six structurally related phthalate esters were below 9%. Some environmental samples were analyzed with satisfactory results.

This work is supported by the National Natural Science Foundation of China (No. 20277005). We here express our deep thanks.

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