A New Generic Enzyme Immunoassay for Sulfonamides

Abstract

The study was directed to the development of generic immunoenzyme assay for sulfonamides. N‐sulfanil‐4‐aminobutyric acid which mimics the common part of sulfonamides was used as immunogenic hapten. The obtained rabbit polyclonal antibodies allowed detection of the N‐sulfanil‐4‐aminobutyric acid in indirect competitive immunoenzyme assay down to 0.03 ng ml⁻¹. The lowest detection limit for the sulfonamides tested, 0.1 ng ml⁻¹, was observed for sulfamethoxypyridazine. Eleven other sulfonamides could be determined at concentrations ranging from 1–37 ng ml⁻¹. Thus, the proposed technique can be used in class‐specific sulfonamides detection in products of animal origin.

Keywords:

• Sulfonamides
• sulfanilamide
• N‐sulfanil‐4‐aminobutyric acid
• ELISA
• selectivity of antibodies
• generic immunoassay

Notes

Financial support was obtained from grant of the Russian Foundation for Basic Research “Influence of structural peculiarities of reactants on the specificity of immunochemical assay of sulfonamides and development of class‐specific techniques for their detection” (#06‐03‐33015)