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Abstract
A fluorescence polarization immunoassay for detection of polycyclic aromatic hydrocarbons (PAHs) was developed. Fluorescein‐labeled tracers based on different PAHs were synthesized and studied in order to achieve a high sensitivity using both monoclonal and polyclonal antibodies. The application of different combinations of antibody tracer pairs allows to separately determine groups of small and large PAHs or to realize a class‐specific assay. The limits of detection of PAHs were 0.9, 1.1, and 3.4 ng ml−1 for benzo[a]pyrene, naphthalene, and anthracene, respectively. The developed fluorescence polarization immunoassay could be used for rapid screening of natural water samples for contamination with PAHs.
The financial supports were obtained from NATO grant “Development of express polarization fluoroimmunoassay of polycyclic aromatic hydrocarbons (PAHs) and its application for screening environmental samples” (EST.CLG.978618) and Grant from Programme EUREKA, OE77 COCANAL