Signal Amplification in Immunoassays Using Labeling via Boronic Acid Binding to the Sugar Moiety of Immunoglobulin G: Proof of Concept for Glycated Hemoglobin

Abstract

A novel electrochemical immunoassay based on the multiple affinity labeling of the indicator antibody with an electro‐active tag is presented. The concept is illustrated for the determination of the glycated hemoglobin HbA1c in hemoglobin samples. Hemoglobin is adsorbed to the surfactant‐modified surface of a piezoelectric quartz crystal. Whereas the quartz crystal nanobalance is used to validate the total Hb binding, the HbA1c on the sensor surface is recognized by an antibody and quantified electrochemically after the sugar moieties of the antibody have been labeled in‐situ with ferroceneboronic acid. The sensitivity of this sensor is about threefold higher than the sensitivity of a hemoglobin sensor, where the ferroceneboronic acid is bound directly to HbA1c.

Keywords:

• Immunoassay
• ferrocene
• boronic acid
• hemoglobin
• QCN
• voltammetry

The authors kindly acknowledge financial support from BMBF (Innoregio 03i1308B). We thank Sophie Haebel for Maldi‐TOF measurements.