Amperometric Glucose Biosensor Based on Ultrafine Platinum Nanoparticles

Abstract

In the present paper the ultrafine and highly dispersed platinum nanoparticles (average size 3 nm) were used for the construction of a glucose biosensor in a simple method. An excellent response to glucose has been obtained with a high sensitivity (137.7 µA mM⁻¹ cm⁻²) and fast response time (5 s). The biosensor showed a detection limit of 5 µM (at the ratio of signal to noise, S/N=3) and a linear range form 0.2 to 3.2 mM with a correlation coefficient r=0.999. The apparent Michaelis–Menten constant (k _m) and the maximum current were estimated to be 9.36 and 1.507 mA mM⁻¹ cm⁻², respectively. In addition, effects of pH value, applied potential and the interferents on the amperometric response of the sensor were investigated and discussed.

Keywords:

• Amperometric enzyme biosensor
• platinum nanoparticle
• glucose oxidase

Acknowledgments

The authors wish to express their gratitude and appreciation for the financial support from the National Natural Science Foundation of China (No. 50671098 and 20473091).