Preconcentration of Rutin at a Poly Glutamic Acid Modified Electrode and its Determination by Square Wave Voltammetry

Abstract

The voltammetric determination of rutin in 0.04 mol l⁻¹ B‐R buffer (pH 4.0) by square wave voltammograms (+0.41 V vs. Ag/AgCl_(sat.)) at a poly glutamic acid modified glassy carbon electrode was found to be several orders of magnitude lower than that on a bare glassy carbon electrode. Rutin can be preconcentrated on the films of poly glutamic acid and presented linear relationship from concentration of 7×10⁻⁷ to 1×10⁻⁵ mol l⁻¹ in 0.04 mol l⁻¹ B‐R buffer pH 4.0. The method was successfully applied to the determination of rutin in pharmaceutical formulation without any pretreatment.

Keywords:

• Antioxidant determination
• modified electrode
• poly glutamic acid
• rutin

Acknowledgments

The authors thank financial support from FAPESP [process no. 03/06598‐3 (DPS) and 04/00111‐8 (MFB)], CAPES and CNPq.