Fluorimetric Determination of Gatifloxacin in Aqueous, Pure and Pharmaceutical Formulations

Abstract

A spectrofluorimetric method was developed for the determination of gatifloxacin. The emission peak for gatifloxacin was recorded at 495 nm upon excitation at 291 nm. The fluorescence process was pH dependent. The dynamic range for the method was 16–80 ng ml⁻¹with detection limit of 3.97 ng ml⁻¹. A linear relationship between the fluorescence intensity and the concentration of gatifloxacin solution was obtained with r ² of 0.9968. The method has successfully applied to the determination of gatifloxacin in pure, authentic and aqueous samples.

Keywords:

• Gatifloxacin determination
• fluorimetry
• pharmaceutical formulations