Spectrophotometry Study of Interaction of Hyaluronic Acid with Methylene Blue and Its Analytic Application

Abstract

The spectrophotometry method for detection of hyaluronic acid was established based on the interaction between hyaluronic acid and methylene blue. The study showed that the electrostatic interaction was the main interaction between them. The linear regression equation of the detection method for hyaluronic acid was ΔA=0.0059+0.046 C_HA (µg/mL), the correlation coefficient, r, was 0.9994, and the linear range was from 0.167 to 4.17 µg/mL. The concentration of hyaluronic acid in the ruijie eyedrop was successfully determined to be 9.83×10² µg/mL. The recoveries were from 95.1% to 104.9%, and the relative standard derivations were from 2.8% to 4.9%.

Keywords:

• Hyaluronic acid
• methylene blue
• interaction
• detection
• spectrophotometry

This work was supported by the National Natural Science Foundation of China (20675013), the Science Foundation of Jiangsu (BK2006090), and Jiangsu Board of Health (Y2006025).