Amperometric Enzyme Biosensor Based on the Controlled Pore Glass

Abstract

A new amperometric biosensor based of glucose oxidase immobilized in aminopropyl-controlled pore glass (CPG) is reported. The glucose oxidase was linked to the CPG by covalent bonds with glutaraldehyde. The effect of analytical variables on the biosensor response was studied using experimental design methodology. Analytical properties such as linearity, detection limit, quantitation limit, range, and precision are reported. Interferences caused by compounds usually present in biological samples were eliminated.

Keywords:

• Amperometric biosensor
• Controlled pore glass
• Experimental design
• Glucose-oxidase
• Validation

ACKNOWLEDGMENTS

The authors acknowledge financial support from the Comunidad of Madrid (Ref. Grupo 950247) program for “Consolidation of Research Groups”. We also thank A. Rodriguez (Electron Microscopy Centre, UCM) for valuable technical and professional assistance.