Development of a Novel Silver Nanoparticles-Enhanced Screen-Printed Amperometric Glucose Biosensor

Abstract

A disposable glucose biosensor is developed by immobilizing glucose oxidase into silver nanoparticles-doped silica sol-gel and polyvinyl alcohol hybrid film on a Prussian blue-modified screen-printed electrode. The silver nanoparticles-enhanced biosensor shows a linear amperometric response to glucose from 1.25 × 10⁻⁵ to 2.56 × 10⁻³ with a sensitivity of 20.09 mA M⁻¹ cm⁻², which is almost double that of the biosensors without silver nanoparticles. The immobilized glucose oxidase retained 91% of its original activity after 30 days of storage in phosphate buffer (pH 6.9; 0.1 M) at 4°C. Blood glucose in a rabbit serum sample was successfully measured with the biosensor.

Keywords:

• Amperometric biosensor
• Glucose oxidase
• Prussian blue
• Screen-printed electrode
• Silver nanoparticles

The support of the Science and Technology Commission of Shanghai Municipality (STCSM; No. 065407061) is gratefully acknowledged.