Analysis of Volatile Compounds of Leaves and Galls of Schinus polygamus and Baccharis spicata by Headspace Solid-Phase Microextraction

Abstract

Headspace Solid-Phase Microextraction (HS-SPME) is presented as an efficient tool to access differences and similarities in the composition of volatiles that might have been caused by insect-plant interaction, using leaves and galls of Schinus polygamus and Baccharis spicata. Method development involved testing nine SPME coatings, resulting in the choice of the triple layer divinylbenzene-carboxen-polydimethylsiloxane (DVB-CAR-PDMS). Qualitative and quantitative differences between distinct plant tissues and also between hydrodistilled oil and headspace chromatographic profile of the same sample confirmed that HS-SPME is a convenient analytical tool to provide information closer to that of the plant in vivo on possible allelochemicals of different plant tissues involved in the insect-plant interaction.

Keywords:

• Baccharis spicata
• Essential oils
• Galls
• Headspace solid-phase microextraction
• Schinus polygamus

We are grateful to Supelco North America for providing the SPME fibers. We also thank CNPq, FAPERGS, and CAPES for providing scholarships to F.C. Damasceno and K.P. Nicolli. Dr. Nelson Matzenbacher is specially acknowledged for helping with S. polygamus sampling.

Notes

^a LTPRI OV5. Linear Temperature Programmed Retention Index in Column OV5

^b LTPRI Lit. Adams´ Linear Temperature Programmed Retention Index (Adams 2001)

^c LTPRI Wax—Linear Temperature Programmed Retention Index in Column Wax

^d Gua—Guaíba

^e Via—Viamão

^f PoA—Porto Alegre

^g SL. sound leaves

^h SPGL—sound part of galled leaves

ⁿⁱnon identified

^trtraces < 0.1%

Compounds in bold letters are the most abundant ones

∗Peak area percentage.