Determination of S-Adenosylmethionine and S-Adenosylhomocysteine from Human Blood Samples by HPLC-FL

Abstract

S-adenosylmethionine plays an important role in many biochemical reactions, including human health maintenance and disease prevention. This paper proposes a simplified, rapid, and easy high performance liquid chromatography (HPLC) with fluorimetric detection method of S-adenosylmethionine and its product, S-adenosylhomocysteine. Determination of S-adenosylmethionine and S-adenosylhomocysteine was by HPLC with fluorimetric detection by the formation of the fluorescent 1,N ⁶-ethanoderivatives of these compounds, which showed a linear concentration range of 310⁻⁶–310⁻⁸ molL⁻¹ for S-adenosylmethionine and 510⁻⁷–510⁻⁹for S-adenosylhomocysteine; the detection limits of the method ranged in the nanomolar domain. The method applied to real plasma samples lead to values of 60.1±19.6 for S-adenosylmethionine and 24±14.1 nmolL⁻¹ for S-adenosylhomocysteine.

Keywords:

• 1
• N ⁶-ethanoderivatives
• HPLC
• S-adenosylhomocysteine
• S-adenosylmethionine

The authors are grateful to Romanian Ministry of Education and Research (research grant CEEX-117/2006 (REN)) for the financial support.