Abstract
In this work, for the first time, the fluorescence enhancement of Ho3+ ions is introduced as a novel probe for human serum albumin (HSA) determination in aqueous solution. Lanthanides express very specific luminescence emissions, due to their unfilled 4fn electronic orbital, and thus, the fluorescence of the lanthanide ions can be radically improved when they are coordinated with the appropriate organic molecules. By applying this method, HSA can be determined up to 0.066 mg L−1.