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Analytical Letters Volume 41, 2008 - Issue 13
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Detection of Aflatoxins in Capsicum Spice Using an Electrochemical Immunosensor

Miroslav Pohanka Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic; Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech RepublicCorrespondence[email protected]
,
Frantisek Malir Institute of Public Health, Department of Xenobiochemistry, National Reference Laboratory for Biomarkers of Mycotoxins, Hradec Kralove, Czech Republic; Pedagogic Faulty, Department of Biology, University Hradec Kralove, Czech Republic
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Tomas Roubal Institute of Public Health, Department of Xenobiochemistry, National Reference Laboratory for Biomarkers of Mycotoxins, Hradec Kralove, Czech Republic; Pedagogic Faulty, Department of Biology, University Hradec Kralove, Czech Republic
&
Kamil Kuca Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic; Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
Pages 2344-2353 | Received 30 May 2008, Accepted 20 Jun 2008, Published online: 05 Nov 2008
 
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Abstract

Electrochemical immunosensor based on immobilized aflatoxin B1 (AFB)–albumin conjugate and polyclonal antibody against AFB1 was performed for a competitive assay of AFB1 in capsicum spice. Spiked samples were used for construction of calibration curve. The proved limit of detection was 2.4 ppb. Immunosensor long-term stability was also estimated. Decrease of immunosensor sensitivity was less than 10% when stored in a refrigerator and approximately 22% for the immunosensor preserved at laboratory temperature for two weeks. Consequent performance of immunosensors for assay of real capsicum spice samples with proven aflatoxins presence produced good correlation with the data from valid method (high-performance liquid chromatography with fluorescence detector).

The grant of the Ministry of Industry and Trade of the Czech Republic (Grant No. 2A-1TP1/009), which supported this work, is gratefully acknowledged.

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