Flow-Injection Chemiluminescent Determination of Piroxicam Using Tris (2,2′-bipyridyl) Ruthenium(II)—Potassium Permanganate System

Abstract

A rapid and sensitive chemiluminescence method using flow-injection has been developed for the determination of an analgesic agent drug, piroxicam. The method is based on the chemiluminescence reaction of piroxicam with an acidic potassium permanganate and Ru(bipy)₃ ²⁺. The chemiluminescence intensity is greatly enhanced when quinine sulfate is used as a sensitizer. After optimization of the different experimental parameters, a calibration graph was obtained over a concentration range of 3.0 × 0⁻⁸–3.0 × 0⁻⁵ mol L⁻¹ with the detection limit of 1.0 × 0⁻⁸ mol L⁻¹. The relative standard deviation is 1.5% (n = 11) for the determination of 8.0 × 10⁻⁷ mol L⁻¹ piroxicam. The proposed method was successfully applied to commercial tablets, spiked serum, and urine samples.

Keywords:

• Chemiluminescence
• flow-injection
• piroxicam
• tris (2,2′-bipyridyl) ruthenium(II), potassium permanganate

The authors acknowledge the support from the National Natural Science Foundation of China, the Science Foundation of Huazhong University of Science & Technology (0101013008), and the Science Foundation of Huaibei Coal Industry Teachers' College.

Notes

UV/Vis: ultraviolet and visible spectrophotometry; CAM: Combining Amperometry and Multicommutation; LC: liquid chromatography; HPLC: high-performance liquid chromatography; LC-MS/MS: liquid chromatography–tandem mass spectrometry method; CL: Chemiluminescence.

^a From Zhuzhou Yashen Pharmaceutical Co., LTD. Zhuzhou, China. (H41022631).

Xi′an No 4. Pharmaceutical Co., LTD. Xi′an, China. (H61021109).

Gannan Pharmaceutical Co., LTD. Gannan,China. (H36021370).

Note. R.S.D relative standard deviation.

^a From a healthy volunteer man in local hospital, Huaibei, China.

^b Mean ± sd, Average of six determinations.

Note. R.S.D, relative standard deviation.

^a From three healthy volunteers men in local hospital, Huaibei, China.