Highly Sensitive Spectrofluorimetric Determination of Trace Amounts of Superoxide Dismutase Using a Prulifloxacin-Terbium(III) Probe

Abstract

A new spectrofluorimetric method was developed for determining superoxide dismutase. The interactions between prulifloxacin (PUFX) –Tb³⁺ complex and superoxide dismutase had been studied by using UV-Vis absorption and fluorescence spectra. Using prulifloxacin–Tb³⁺ as a fluorescence probe, under optimum conditions, superoxide dismutase could remarkably enhance the fluorescence intensity of the prulifloxacin–Tb³⁺ complex at λ = 545 nm, and the enhanced fluorescence intensity was in proportion to the concentration of superoxide dismutase. Optimum conditions for the determination of superoxide dismutase were also investigated. The dynamic range for the determination of superoxide dismutase was 0.032 to 22.56 µg mL⁻¹, and the detection limit (S/N = 3) was 1.5 ng 4 mL⁻¹. This method was simple, practical, and relatively free of interference from coexisting substances and could be successfully used to determine superoxide dismutase in the plant and blood samples. The mechanism of fluorescence enhancement of prulifloxacin–Tb³⁺ complex by superoxide dismutase was also discussed.

Keywords:

• Dismutase
• luminescence
• mechanism
• prulifloxacin
• spectrofluorimetry
• superoxide
• terbium

The authors acknowledge the support from the National Natural Science Foundation of China, the Science Foundation of Huazhong University of Science & Technology (0101013008), and the Science Foundation of Huaibei Coal Industry Teachers' College.

Notes

Note. RSD, relative standard deviation.

Note. RSD, relative standard deviation.