Analysis of Chlorpyrifos in Water, Fruit Juice, and Honeybee Extract by Chemiluminescent Elisa

Abstract

The suitability of competitive enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection-based immobilized antigen (indirect assay) for rapid and accurate determination of chlorpyrifos in various food matrices was tested. The limit of detection (LOD) values were 1–1.75 ng mL⁻¹, the standard curve midpoint (IC₅₀) was 3.5 ng mL⁻¹, and the assay duration was 1.5 h. Assay application to the analysis of honeybee extract resulted in chlorpyrifos recoveries varying between 62 and 83% in 5–15 ng mL⁻¹ herbicide concentration range.

Keywords:

• Chemiluminescence
• chlorpyrifos
• Elisa
• food/fruit juices
• honeybee

Notes

^a Each standard was prepared independently.

^b precision intra-assay as coefficient of variation (n = 3).